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Bee Lab

Cognitive Bee Lab

Locations:

  • Behaviour Chamber: Fir 223, Surrey campus
  • Virtual Reality Tunnel: 3074, Richmond campus

Current Projects

Honours Thesis: “A marker-based closed-loop capable virtual reality system for bees”

Student Researcher: Tina Charmchi (B.A. Honours, Psychology)

Bee Virtual Reality TunnelMany species of bees are ideal as animal models for the study of visual guidance and navigation related questions because of their convenient, lab-friendly life-cycle and very capable brains. Here, we report on our design of a new marker-based, high-throughput virtual reality system (for markerless systems, see Stowers et. al., 2017). The current system is open-loop, but all the necessary hardware components are present to operate as a closed-loop system through a software customization. The two components of the system are tracking and rendering. Tracking the 3D position of flying bees is accomplished with six OptiTrack Flex13 USB cameras, which compute bees’ positions with an 8.3 ms latency. Rendering is comprised of four 55-inch television displays (9.1 ms input lag), which are configured in a 2.44 m V-shaped tunnel. The system is driven by a computational server with a 3.6 GHz octa-core processor, which handles tracking, and rendering with a GeForce 1070 Ti high-performance video card that has four simultaneous outputs. The V-shape of the tunnel allows for the presentation of stimuli in both ventral and lateral visual fields. Virtual reality allows the rendering of arbitrary space configurations making it possible to, for example, have bees experience a rectangular tunnel. The key advantage of this system is that it increases experimental control while also increasing external validity, tracking is accomplished with very low latencies, and the system is very easy to set up. Data from our first project manipulating horizontal bars as in Dakin et al, (2016) will also be presented.


"How bumblebees (Bombus impatiens) use visual cues of conspecifics under stressed and rewarded internal states"

Student Researcher: Jaime Christiaanse (B.A., Honours, Psychology)

12-arm Radial Maze Bee LabBumblebees are social animals that use visual information about other bees to choose flowers (Leadbeater & Chittka, 2009). Workers prefer flowers that are occupied by another bee when the occupier is large relative to the flower, and when occupied flowers are relatively rare in the field compared to empty flowers (Plowright et. al., 2013). It is unclear whether this preference is due to workers viewing others as competitors, signaling a flower has been depleted of its food resources; informers, signaling the flowers that are abundant in food resources; or cheaters, looking to steal rewards from the occupier (Lihoreau, Pasquaretta & Heeb, 2016; Service & Plowright, 2002). Stress also influences food-finding behaviour and decision making in bumblebees and honeybees (Bateson, Desire, Gartside, & Wright, 2011; Perry, Baciadonna, & Chittka, 2016). It is not known whether the preference for occupied flowers, and therefore, social learning in general, is modulated by workers’ internal states; whether it be stress or an unexpected positive reward (Bateson et al., 2011; Perry et al., 2016). Here, we investigate reasons under which the preference for occupied flowers varies with the internal state of B. impatiens workers. To manipulate the workers’ internal state, we present workers with a droplet of 1 M rewarding sugar solution, or 1 mM aversive quinine solution prior to releasing the worker into a 12-arm radial arm maze. Data collection is ongoing, but currently, workers’ choice proportions do not significantly deviate from the expected values in both quinine (Gp = 0.80, p = 0.37, n = 9) and sugar (Gp = 0.66, p = 0.42, n = 7) conditions. There was a significant difference between preferences for occupied and empty flowers in sugar exposed workers (p  = 0.005, GP = 8.025, GH = -58.697). There was not a significant difference between preferences for occupied and empty flowers in quinine exposed workers (p = 0.130, GP = 2.297, GH = 298.054). These results suggest that quinine removes the preference for occupied seen in the sugar exposed workers and in previous research. There was not a significant difference between the corridor a worker was facing at the beginning of flight and the first choice (p = 0.231). This result suggests the first choice was dependent on the angle of departure, and the angle of departure was random because workers could not see the flowers before they began flying due to a lip blocking the view from the maze stage, as well as the partitions between the corridors.